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Frozen sections of various brain lesions were thawed to room temperature, washed in PBS, permeabilized with 0.3% Triton X 100 and blocked for non-specific binding sites in 3% skimmed milk powder in PBS for 1 hr.
Over the last few years, frameless stereotactic biopsies have progressively replaced frame-based stereotactic procedures as a first line diagnostic approach to various brain lesions.
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Effect of pharmacological challenges Studies with C-labelled AMT in experimental animals using autoradiographic techniques after various interventions and brain lesions indicated that AMT could detect changes in the rate of 5-HT synthesis (see reviews by [ 31, 54]).
> -wrap-foot> All cases underwent detailed neuropathological examination on multiple tissue blocks from various brain regions and lesion activity was evaluated as described in detail previously (Frischer et al., 2009).
To analyze the relation between kainic acid-induced limbic seizures and the associated brain lesions, various doses of kainic acid (117-940 pmol) were administered intracerebroventricularly to unanesthetized rats.
In addition to impairment of cerebral blood flow and its determinants, cerebral microcirculatory dysfunction has also been shown to be associated with brain lesions in various experimental models of sepsis but not yet in humans [ 1, 24], although it may probably be linked to diffuse ischemic damage and microhemorrhages observed in brains of patients who died of septic shock [ 22].
Presumably, pathological changes and lesions involving various brain areas and neuronal networks lead to changes in the functioning of the brain, which in consequence affect memory, activities of daily living (ADL) and the behaviour of patients [ 1, 2].
It is important to note that different measures of motoric impulsivity are differentially affected by lesions to various brain regions, once again suggesting that impulsivity is non-unitary in nature.
Patients with various types of traumatic and nontraumatic brain lesions also appear to profit from this approach [ 8].
In addition to a hypothesis of indirect propagation of PrPSc into the CNS, various combinations of clinical manifestations and brain lesions may influence the symptoms of dCJD patients.
Effect of cerebellar lesions on monoamine levels in various brain areas of the cat.
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