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The ACT-Graph can be used to identify various alternative splicing features in a gene.
Fig. 5a is a schematic of the various alternative splicing (AS) events that were analyzed and the number of events identified are listed in Fig. 5b.
This extreme diversification of the DMRT1 structure was accompanied by various alternative splicing options such as the inclusion of introns, exon skipping, the occurrence of pre-stop codons, and frame-shift mutations (Supplementary information, Figure S15B-S15D).
Unexpectedly, various alternative splicing events were found in the processing of polycistronic transcripts from the snoRNA gene clusters I and II by analyzing cDNA sequences from RT-PCR of the transcripts.
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In the context of exon array analyses, we define the overall gene expression index as the total abundance of all molecules transcribed from a single gene, including various alternative splice forms, alternative 5' transcripts, alternative poly A transcripts, etc.
It is also planned to incorporate the corresponding DNA data and to track the various alternative splice forms of the proteins.
Datasets of spliced alignments from the TIGR [ 6, 10] and RIKEN [ 11] databases of full-length cDNAs and ESTs provide useful annotated versions of the Arabidopsis genome sequence for the detection of various alternative splice events.
However, most genes are expressed in various forms by alternative splicing (AS), extending transcriptome complexity.
HIV, a lentivirus belonging to the Retroviridae family of viruses, has a ∼9.6kb diploid RNA genome, encoding nine genes and at least 15 protein products, resulting from various mechanisms including alternative splicing, ribosomal frame shifting and proteolytic cleavage of the proteome [2], [3].
This shows various types of alternative splicing, including alternative promoter usage, cassette exons etc. that result in more than a single transcript.
Tο investigate various isoforms with alternative splicing of exons 11 to 13, we used forward primers located in the middle of exon 10 and reverse primers located in the middle of exon 13.
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