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Why was variegated expression seen only for weakened enhancers?
We sought therefore to generate variegated expression with simple transgenes and then to identify molecular requirements for variegated expression.
Of particular interest, a segment that was wholly derived from the bacterial gene yielded variegated expression.
We conclude that variegated expression requires a weakened, but non-zero, enhancer.
The gpt-derived segments that yielded variegated expression were, of course, non-physiological.
These features suggested that this system might be suitable for identifying conditions for variegated expression.
Similar(6)
This observation suggests that dCTCF is required for the non-variegated expression of the garnet gene when it is maternally inherited.
In addition to the strong influence of epigenetic regulation, another important aspect of FSHD1 contracted D4Z4 regions is the variegated gene expression of DUX4-fl mRNA, as both traits are characteristic of metastable epialleles.
Metastable epialleles (reviewed in [ 43, 44]), as opposed to traditional alleles, have variable expressivity leading to phenotypic mosaicism between individuals, as well as variegated cellular expression leading to phenotypic mosaicism between cells.
This was done in an effort to circumvent variegated transgene expression resultant to methylation-induced silencing of UAS-based transgenic lines in zebrafish, specifically demonstrated to effect expression of the Tg(14xUAS:nfsb-mCherry c264 nfsb-mCherry c264
Due to variegated transgene expression, the percentage of Venus+ cells was determined from peripheral blood and only mice with >80% of Venus+ cells in F2 were used for further breeding with non-transgenic mice to maintain a hemizygous state.
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