Sentence examples for variations were validated from inspiring English sources

Sequence traces were analyzed using the SeqScape v2.5 software (Applied Biosystems), and all sequence variations were validated by re-sequencing independent PCR products.

Sanger sequencing of PCR products using genomic DNA of the samples was conducted, and 99/108 (91.7%) tested structural variations were validated.

The nsSNP variations were validated with a pair of primers as described (Additional file 10) and subsequently used for a high-resolution melting (HRM) analysis using the Kapa HRM Fast PCR Kit (Kapa Biosystems, Boston, Massachusetts, USA) and Eco Real-Time qPCR System (Illumina, San Diego, California, USA) according to the manufacturer's instructions.

The analytical dispersion relations, in the presence of the spatiotemporal material variations, are validated numerically using finite 2D membranes with a prescribed number of cells.

Recently, an analysis method aimed at making the results independent of the quality of the shim and interindividual variations was validated by biopsy in 149 patients [ 17].

The web-based database dbSNP (http://www.ncbi.nlm.nih.gov/projects/SNP/) was applied in the confirmation of known SNP and the novelty of detected nonsynonymous variations was validated by web-based database Catalogue of Somatic Mutations in Cancer (COSMIC, http://cancer.sanger.ac.uk/cancergenome/projects/cosmic/).sanger.ac.uk/cancergenome/projects/cosmic/

CAI variation was validated with three stations sampled with 30 other photos.

Protein abundance variation was validated by Student's t-Test (p < 0.05).

Importantly, robustness to inter-visit variation was validated by a second data set derived from the same tumours.

We speculated that the joint effect of the genetic variations, which were validated in our study, provided a more strong association with risk of T2DM and IGR.

All DNA variations identified were validated using a second independent DNA sample.