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The other 5 detected variations were synonymous (Table 1).
In addition, the distribution of synonymous and non-synonymous changes in the MCP gene revealed that the iridoviruses were evolving slowly, and most of the variations were synonymous mutations.
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The other 5 variations are synonymous and only one of them is novel (p.Pro48Pro).
We indicate the same phenomenon here, with nominally 30 identified expressed genes (and ~130 anonymous truncated transcripts) present as up to 28 variants, for which the majority of nucleotide variation was synonymous.
The exonic variations that were synonymous or present in dbSNP with minor allele fraction ≥ 5% were filtered out.
Most of the variations observed were synonymous mutations, and the nucleotide substitutions in these isolates occurred at the first and second nucleotide positions and did not cause amino acid changes.
The DNA sequence study revealed nine nucleotide changes in the L1 gene, two of which (2/9) were nonsynonymous variations and 7/9 were synonymous variations.
Despite the sequence variations, nearly all nucleotide exchanges were synonymous.
Two sequences of Hd6 derived from the different genome type of tetraploid O. minuta revealed nucleotide variations at two sites, but both were synonymous substitutions.
Of the 34 SNPs detected, 22 variations belonged to nonsynonymous and 12 were synonymous mutations.
This finding indicates that most nucleotide variations in N gene coding sequence were synonymous mutations compatible with ongoing natural selection.
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