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Small variations were indeed seen in the expression of the traditional housekeeper genes (see Table 2), while the 4 selected invariant genes showed consistent expression across both rat lines and in both cohorts (see Figure 2 for examples).

Furthermore, time-course experiments demonstrated that the observed variations were indeed related to the differentiation process and did not depend on other phenomena such as exit from cell cycle or senescence owing to prolonged time culture (Supplementary Figure 2E).

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To ensure that these 134 "H37Ra-specific" variations are indeed unique to H37Ra and not due to possible sequencing errors of H37Rv sequence, we again re-sequenced these 134 "H37Ra-specific" variation loci using our strain of H37Rv (obtained from Trudeau Institute but maintained in mice, see Methods).

Such variation was indeed present in the synthetic dataset with differential gene expression (Fig. 1k,n), and hence we can hypothesize the same for the real dataset (Fig. 1j,m).

If this variation is indeed random, it may be an evolutionary strategy to promote diversity.

Since repeat complexity and repeat count (in case of complex STRs, the repeat count of the longest homogenous array) have also been reported to influence STR marker variation [7], we analysed our markers according to these features in order to ascertain whether the difference observed between tri/tetra and penta/hexa marker variation was indeed due to repeat unit size.

Thus, as predicted by theory, the extent to which a mutant increases phenotypic variation is indeed correlated to both the fitness effect of the knockout and to the number of severe negative genetic interactions of the mutated protein, while other tested variables appear to be unimportant.

Although, individual variation was, indeed, observed for the Paria population in an earlier study [ 25].

Another important question is how within-taxon variation of hybrids compares to that of its parents – if this variation is indeed a key determinant of success.

Second, the cancer risk conferred by the genetic variation is indeed very modest, and the penetrance is very small for the variants.

Variation is indeed a key tool in grounded theory, for it breaks through narrow specification to broaden the scope of theory.

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