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Variations of parasite density were assessed by quantile regression using bootstrapping with 1000 repetitions.
Moreover, large inter- and intraobserver variations of parasite staging are frequently observed (7).
A study defining phenotypic variations of parasite isolates at the point of clinical diagnosis could allow us to predict the evolution and the outcome of disease.
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Parasitological studies on the other hand, revealed an annual prevalence of 35% of Plasmodium falciparum asexual parasites carriers, a seasonal variation of parasites prevalence in the population and, a potential infectious reservoir dominated by the age group ranging from 0 – 15 years [ 8].
Despite this variation, rates of parasite growth in these volunteers were similar.
We also calculated the agreement between the genotypes following supertype classification (see Table 3, last column), since supertypes will be considered as the unit of selection in subsequent analyses investigating the effects of Mhc variation on parasite burdens, survival, reproductive success and mate choice in this system.
Such genome stratification approaches will help monitor variations of malaria parasites within South Asia and future changes in parasite populations that may arise from in-country and cross-border migrations.
Longitudinal studies are needed to assess the variation of asymptomatic parasite carriage over time, and its exact contribution to transmission.
Such approaches will benefit from a better understanding of the genome variation of this parasite.
Previously, we quantified the proportion of the phenotypic variation of four parasite resistance traits that are under genetic control [ 9].
The present study has provided many new candidate genes, including putative antigens that can provide starting points for functional investigation of phenotypic variation of these parasite lineages.
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