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The variations observed here for some acoustic parameters would have likely been decreased and the differences between morphs would have likely been built up by studying individuals coming from different colonies for each morph.
The variations observed here may be attributed to cultural differences between China and USA.
Alternatively, evolution of these behavioral phenotypes may involve only a few genes or pathways to achieve the behavioral variations observed here.
Although our experimental design did not allow segregating between the quantity of xenobiotic and their inherent chemical properties, it is likely that molarity and lipophilicity are key factors affecting the magnitude and the specificity of transcriptome variations observed here.
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The intra-individual variation observed here resembles variability of tDCS effects in motor regions and may be one reason why in this study robust tDCS effects at a group level were missing.
It is conceivable that the temporal variation observed here reflects dynamics within a larger meta-community and might not be observed in outbred populations with non-sterile food.
A lack of between-season agreement would not have been surprising in itself; it is the pattern of seasonal variation observed here which was unexpected.
The acoustic variation observed here is not consistent with the pattern of harmonic hopping observed by Kingston and Rossiter [ 29] in the R. philippinensis complex, but is similar to divergence patterns in the H. bicolor complex [ 33].
Haplotype networks indicate that red JF is the primary contributor of diversity at chicken IL-4Rα: the signature of variation observed here may be due to the effects of domestication, admixture and introgression, which produce high diversity.
However, artificial (directional) selection during the early domestication process would have caused a drastic loss of diversity, which in no way accounts for the high degree of variation observed here.
The seasonal pattern of nuchal crest variation observed here, could therefore just be a consequence of the unnaturally nutrient rich captive environment in which domestic native breeds are kept.
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Justyna Jupowicz-Kozak
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