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Using T\aguchi methods, we were able to evaluate the effects of four variations (levels) in each of these five parameters, with just 16 experiments (an L16 (1024) orthogonal array).
Since all cells from an individual have the same DNA sequence variations, levels of gene expression in immortalized cell lines can reflect the functional effects of DNA sequence variations that influence or regulate gene expression.
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A central composite experimental design with five variation levels was chosen.
The process variables, FT and Ft, had variation levels of 31 36°C and 48 72 h, respectively.
A central composite experimental design with five variation levels was used and response surface methodology applied for the data analysis.
The effect on chitosan DA was investigated using a fractional factorial design 27−3 with seven factors and two variation levels.
The relations function in smoothening variation levels.
Independent variables with three variation levels are listed in Table 1.
To apply this design, the variation levels for each variable must be specified clearly.
Also, the roles of the effective parameters and the variation levels of all moduli at same conditions are determined.
The analysis was done at county level and took into account seasonal variation, levels of poverty and even the news of celebrity suicides that can lead to more deaths.
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