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In addition, structures with variations in the side chain conformations were generated for each model; however, the first side chain model was always built with the UQO procedure.
While DAOs show absolute selectivity for conversion of R-configured α-amino acids, these enzymes tolerate a wide range of structural variations in the side chain of their substrates.
Further work has revealed the heterogeneity of the toxin from different geographical sources, with structural variations in the side chain that could be traced to specific changes in the biosynthetic pathway.
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Stepwise removal of cationic substrate non-prime side residues and variations in the prime side sequence resulted finally in an uncharged tetrapeptide, WYCW-NH2, with inhibitory Ki values of 4.2, 4.8, 24.4, and 11.2 μM for the DEN-1 4 DEN-1 4es, resproteases.
Small variations in the compound side chain length and position changed the binding preferences for individual enzymes.
As shown in Figure 2, the three dihydropterin analogues retain the ring structure of H4B and introduce variations only in the side chain.
Other than length, further structural variation in the side-chain includes the presence and orientation of hydroxyl groups, the presence of unsaturation in the side-chain, and above all, the substituent on the terminal carbon atom, which is a hydroxyl group in bile alcohols and a carboxyl group in bile acids.
The presented method also considers and properly addresses the dc-voltage variations in the dc-side of the interfacing system.
The truth is that Madrid's coaching staff have indeed thought about systems and variations with Bale in the side and the question was a legitimate one.
The results indicated the regions of the epitope that suffer a restraint and those that could accept variations in the amino acid side chains.
When these were coupled with incomplete methylation of 1 amino acid and natural variation in the lipid side chain, 18 lipopeptides were generated.
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