Specifically: (1) the model revealed the possibility that under conditions of Lis1 reduced expression, neurons experience an oscillatory neuron-glial association prior to the multipolar stage; and (2) we hypothesized that observed morphology variations in rats and mice may be explained by a single difference in the way that Lis1 and DCX stimulate bipolar motility.
We therefore speculate that a subtle effect on APH1B expression or function (copy number variation in rat or Phe217Leu variation in human, respectively) may affect γ-secretase activity and signalling downstream of gamma-secretase cleavage and, consequently, give rise to vascular complications.
The specificity of this effect is supported by the observation that intrathecal BoNT-B failed to have any effect after delivery in rats, a finding consistent with a single amino acid variation in rat VAMPI protein rendering resistance to BoNT-B cleavaging activity.
The variations in the rat NF-1 protein (compared to the human sequence) are not located within its DNA binding domain, thus they are unlikely to affect its binding to DNA.
Although the brown rat (Rattus norvegicus) is widely used as a model mammal throughout biological sciences, little is known about genetic variation in wild rat populations or the relationship of commonly used inbred strains to their wild relatives.
Only one CRG, rev-erba, showed 24-hour variation in fetal rat liver (E20) [42], consistent with the lack of rhythms in E18-19 fetal mouse liver reported here.
Taken together, the observed variation in the rat mitochondrial tRNA-Cys might not only affect the role as a tRNA but also affect priming of L-strand replication.
The addition of these novel variants significantly expands known variation in the rat genome.
Furthermore, we identified substrain variation in seven rat strains and find that the degree of variation is highly variable between strains.
No significant variation was noted in rat's body weight at the end of 14 days of experiment between groups.
