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Inbred mouse strains are used in forward-genetic experiments, designed to uncover genes contributing to their highly distinct neurophenotypes and multiple reports of variations in mutant phenotypes due to genetic background differences in reverse-genetic approaches have been published.
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One possibility, which we investigated and could reject, is variation in mutant hINS gene expression in different DGRP backgrounds.
There was no substantial variation in mutant loads assessed at various stages of pregnancies until birth (Table 4).
However, Li et al. [ 38] performed whole-exome sequencing of 66 individual cells from a single muscle invasive tumor, and identified large variation in mutant genes between the cells.
In addition, new genetic variation in mutants can be exploited in traditional plant breeding [ 6, 11].
We speculate that the folding landscape of a micelle is rougher than that of a bilayer, and more accommodating of conformational variations in nonoptimized mutants.
The vectorial variations in the mutants may indicate a decreased tubule stability and vulnerability to distortion.
The locations of the true positive variations in the B. anthracis mutants are listed in Table 2, while the true positive variations in parent strains are listed in Table S1.
They developed cytogenetic tools that include long lists of meiotic mutants, variations in chromosome type and number, and ultimately over a thousand translocation lines (Anderson 1956).
The presence of diverse subgroups within the LytR-CpsA-Psr family might indicate functional differences, and could explain variations in phenotypes of respective mutants reported.
In the study reported here we have used DNA microarrays and metabolic profiling to obtain more information on ways in which plants respond to variations in FDE capacity, using mutants with no or overexpressed levels of PsbS.
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