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Here we present an optimized procedure to generate amino acid variations at specific site(s) of proteins, followed by a simple one-step screen for mutants with the desired β-glucosidase activity.
Liu et al. (2008a) investigated the seasonal variations in the O 1S) and OH nightglow emission rates at mid-to-high latitudes using WINDII/UARS data and showed that both emissions showed semi-annual and annual variations at specific altitudes and local times.
Genotyping techniques determine predefined genetic variations at specific locations or ?hotspots?
In-depth analysis of the available sequence data on SARS-CoV also revealed that the viral isolates could be readily subclassified into several major genotypes based on nucleotide variations at specific genomic positions [ 8, 12].
Thus, the changes induced by MDV infection likely involved subtle variations at specific genomic loci rather than de novo establishment or abrogation of histone marks.> -wrap-foot> Merged peaks are filtered by removing those appearing in only one sample.
The global LINE-1 assay with degenerate primers did not show this tendency (Supplementary Material, Table S1) probably due to the insensitivity of the assay to variations at specific loci or because only a subset of full-length L1Hs LINE-1 elements studied here are showing this tendency.
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This procedure decomposes the spatial relationships among sampling sites into components, the eigenvectors or principal coordinate axes, which reflect variation at specific spatial scales.
Overall, this approach provides an accurate and robust method for the confirmation of tissue-specific expression variation at specific loci identified by a microarray experiment or other high-throughput system.
While DNA methylation variation at specific loci, such as imprinted genes, genes on the X-chromosome and transposable elements has been reported [13] [17], interindividual differences in DNA methylation for other genes in human tissues is less well-studied.
Hence intra-individual variation at specific sedation levels is important.
Another form of genetic variation is sequence variation at specific nucleotide sites in protein-coding genes.
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