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In total, 146 repeatable amplicons with length variation were screened from 28 microsatellite primer pairs (Table 2) in 21 species (Table 1).
Although some hallmarks related to virulence variation were screened, the variation mechanism at the transcriptional level was not deeply understood yet.
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Genetic variation was screened at two mitochondrial DNA genes: a 515 bp fragment of cyt b and the complete NADH-1 (ND1: 975 bp) by polymerase chain reaction (PCR) for 4 20 fish per population (Table 8).
The present study is a two-stage analysis: we used a genome-wide genotyping array in the first set of subjects (stage 1) primarily to verify the homogeneity of our population by several statistical analysis like PCA, homogeneity test, and to perform imputation as a huge number of nucleotide variations were screened simultaneously.
Nine variations were screened by SSCP analysis, three were screened using an Applied Biosystems TaqMann assay and six were screened by automated DNA sequencing.
Libraries of thin films of polystyrene on gradient etched silicon substrates containing orthogonal continuous variation of thickness were screened for dewetting behavior using automated optical microscopy.
Structural variations (SVs) in OSCR11 were screened using the CLC Genomics Workbench 5.1 with the following parameters: P-value threshold, 1.0E−4; paired-read orientation, yes.
The newly sampled individuals were screened for variation at nine microsatellite loci as in [13], except that the Gal263 locus was not included in the analysis.
The oak samples were screened for variation at 19 nuclear SSR loci that had been developed for other oak species [44] [49] (see Table 2 for primer details).
Individuals from Florencia were screened for variation at thirteen microsatellite loci.
These loci were screened for variation in individuals from populations in southwestern Australia.
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