2D data viewer was augmented with a dynamic time slider to visualize variation of readings over time (i.e. requirement 8 in Additional file 1: Table S1), based on the feedback of engineers.
Bland-Altman plots were generated for each reader and showed considerable variation between readings on the two occasions.
19 20 Again, the authors repeated RBT measurements when the variability indicator was high or IOP >19 mm Hg with medium standard variation, excluding readings with erroneous device performance.
We derived the within-plate variation from readings of the positive DHT controls as a coefficient of variation (CV), with 95% of all CVs falling between 2.1% and 12.9% (mean, 6.5%).
Of the 41 MD progeny which were sampled 2 4 times, 12 progeny had > 40%% variation between readings, suggestive of chimeric tissue (both "2n" and "n" chromosome number in sectors of the same plant) (Additional file 5: Table S4).
While this is not unexpected in Brassica progeny derived from microspore culture [ 36], a high level of variation between readings was observed in most higher-ploidy MD progeny, but not in established species controls (Additional file 5: Table S4).
Variations in readings, for a given subject were NCT (± 2 mmHg), ICare (± 1 mmHg) and GAT (± 1 mmHg).
The exclusion of individuals with comorbidities (like autism and Attention Deficit Hyperactivity Disorder) may also be advisable, reducing non-reading specific variation in reading ability attributable instead to comorbid disorders.
There was more variation among the readings from the VNIR instruments than among the readings from the Minolta instruments, probably due to slight differences in optics and geometry among the VNIR instruments.
The error bar represents the variation in the readings.
