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Interestingly, bands from GLC gradually appeared with a slow increase in intensity as the laser irradiation time increased, while the intensity of DMF bands decreased slowly, but the variation rates for bands from GLC and DMF were different.
To rigorously determine if nucleotide variation rates for both the LTR and NRR flanking sequences were associated (and to which extent) with their positions along the Chr3S and G+C content, we used a multiple linear regression model.
For verification, the numerical results are compared with the experimental data according to the various stamp geometries and pressure variation rates, for which a maximum difference of 10% is indicated.
where MIN D,E) is the smaller of D and E. In conclusion, the variation rates for a, e, and i are approximately given by Equations (33) to (35) for i≤H/2a, Equations (36) to (38) for the intermediate inclination (H/2a<i≤2H/a), Equations (30) to (32) for i>2H/a.
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A similar variation rate for A. versicolor might explain the observed difference in Cq values between different strains of this species.
The average variation rate in the annotated NOD Idd regions that resulted from our BAC-based sequencing was ∼22% greater than the variation rate for the same regions calculated using the NOD/ShiLtJ sequence of the MGP.
Using the mathematical model developed by Saunders, et al. [ 35], the maximum phase variation rate for these loci, assuming equal forward and back mutation rate and no fitness difference, is 6.7 × 10-4 per generation.
Therefore, a true per-base variation rate for our bison versus the taurine reference is likely higher than that suggested by our percent of total differences given by the alignment (0.45%, or about 5 per 1000 bases).
This translates to a variation rate of 2.5×10−4 locus−1 year−1 for microsatellite loci, significantly higher than what we measured for nsSNVs.
The variation in mutation rate for the tRNA suppressor mutations can also be explained as variation in the effectiveness of mismatch repair.
CI, confidence interval; CV, coefficient of variation of the aOR; VR MI, variation rate of the aOR for CC and MI analyses; VR SA, variation rate of the aOR for MI and senstitivity analyses.
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