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In future studies, it will be important to further test this correlation in the normal population, using either STX1A gene variation or expression.
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Considering that no obvious SNP mechanisms were determined or differential expression was observed, our results indicate that genomic structural variation or gene expression data alone is not adequate to establish any of these genes' candidacy or causality in relation to regulation of physical activity.
Identification of unique recurrent single nucleotide variations (SNVs) or expression alterations that enable resistance can offer insights into the mechanism of action or cellular targets of compounds.
Other genetic variations such as microsatellites, copy number variations or gene expression levels were not considered.
If the PSR shows reasonable variation or difference in expression between conditions, the posterior probability of measurability should be near certainty.
We compared the average percent of variation (or scatter) in expression of liver transcripts between cohorts 1 and 2 (liverpairs 1/2), cohorts 1 and 3 (liverpairs 1/3) and cohorts 2 and 3 (liverpairs 2/3).
Moreover, tissue-specific databases, such as MGEx-Tdb, also have the potential to assist in exploring the variation or conservation of expression of genes across different species in multiple tissues.
For example auto-regulatory loops can act to reinforce and maintain a factor's expression once activated, while negative auto-regulation results in the repression of a gene by its own product, which can reduce cell-to-cell variation or 'noise' in expression 63, 64.
It follows that variation in the structure or expression of E3 ubiquitin-protein ligase, ubiquitin conjugating enzyme (E2) or other enzymes involved in the ubiquitin proteasome pathway, could be important in affecting the resistance or susceptibility of P. monodon to WSSV.
First, no intraspecific variation in gene content or expression patterns were identified across the multiple analyses of this study (which used three isolates, see Materials and Methods) and published sequence data (table 1), suggesting that the genetic basis of nutritional interactions in this symbiosis in B. tabaci MEAM1 may be relatively uniform.
To characterize complex patterns of genetic variations or RNA/protein expressions in surging Omics data sets, bioinformatics researchers have developed new data analysis approaches gene-set approaches gene-setl., 2009; Nanalysisim, 2008) anDinutwork/pathway analysis (Wu et al., 2009).
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