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Although the dose of NTG necessary to induce a significant drop in PWT (15 mg/kg), was somewhat higher than used by Bates et al. [14], this may be explained by differences in the source of NTG, or by the variation in the tests used to evaluate thermal nociception (Hargreaves test vs. ITHT).
As there was considerable variation in the tests performed and reference standards used in local clinical practice, all completed questionnaires were reviewed centrally by an expert panel of clinicians who determined whether a child had CAH or not on the basis of the clinical presentation and investigations reported by local clinicians.
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24 These variations in the selection of diagnostic tests and cutoff values can have significant clinical and public policy implications.
Our findings help to explain some differences between women choosing or declining early and late screening, but not the large variation in the test uptake among practices, nor between the Netherlands and other countries.
However, this method may provide confusion due to the variation in the test data.
Further variation in the test series was introduced via addition of openings near the slab column intersections.
Another challenge concerns the estimation of the effect of test month on achievement, because typically we do not observe much variation in the test month.
Due to the limited test size and the coefficient of variation in the test standard, it is difficult to draw conclusions regarding the relative efficacy of approaches that were applied to this challenge.
Though informants' responses indicated dominant uniformity at the general level, the survey identified much variation in the testing practice of the six examination boards at the more specific levels, in particular in the areas of pretesting, marking, test equating, test use, etc.
Therefore, and in view of the great variation in the test scores, we rated the agreement as doubtful.
In total, 19 principal components or nine factors were needed to explain the genetic variation in the test dataset.
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