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Variation in the spacer length resulted in submicromolar α-keto-oxazole FAAH inhibitor (IC50 = 436 nM) presenting electrostatic stabilizing interactions between its polar head group contained in the C5-side chain and the hydrophilic pocket of the enzyme.
Of the 102,359 consensus DR2 elements located in Alu repeats, 92,686 corresponded to the sequence AGGTCAnnAGTTCA, and of these, AGGTCAggAGTTCA accounted for 82,319 occurrences, along with 9,877 copies with only one variation in the spacer nucleotides (data not shown).
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Variations in the spacer residues between the chelator and the Trp-Met-Asp-Phe-NH2 influence the affinity only to a minor extent [4].
Relatively small variations in the 3' spacer sequences of the T7 primers immediately reduce the phenomenon dramatically.
Sequence variations in the spacer regions separate them into two groups.
Consistent with this notion, our (in vivo) spacer sequence experiments show that the I-TevI linker can tolerate substantial variation in the DNA spacer, consistent with previous in vitro profiling (Bryk et al. 1993).
At the same time, however, the incomplete tolerance of Tev-mTALENs to variation in the DNA spacer between the TALE binding site and the cleavage motif that we observed highlights the need for additional study.
Genes that have many mutations in coding regions (relative to the reference plastome) may also be missed by Plann, although sequence variation in the intergenic spacers will not affect Plann at all.
Further identification of Symbiodinium spp. type was determined by length variation in the Internal Transcribed Spacer 2 (ITS2, nuclear rDNA) and length variation in domain V of the chloroplast large subunit ribosomal DNA (cp23S-rDNA).
We moreover suggest that variations in T7 spacer sequences introduce platform specific bias that may hamper the data comparison between platforms.
The DR length was 28 to 36 bp for all three strains (it appears the smaller direct repeats are truncated versions of the larger direct repeats), while there was variation in the number of spacers present within each CRISPR (Additional file 13).
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