The hypothesis that restriction modification systems influence the ability of different S. aureus strains to accept SCC element is also supported by data from Waldron and Lindsay [8], who showed that variation in the restriction modification system corresponded with the 10 major S. aureus lineages.
Variation in the restriction digestion patterns of Tragopogon dubius was evident for a single marker (TDF72.3).
Possible frame-shift mutations and phase variation in the restriction and modification genes, which could also be explained by sequencing errors, were not classified as disrupted (Budroni et al. 2011).
Hybridization studies with a range of Bacteroides CTn's encoding tetracycline resistance revealed a great deal of homology between most of the CTn's but there was much variation seen in the restriction patterns of these elements, suggesting great diversity among this group.
As a result, the Precision of the analysis method is more robust to variation in these restriction conditions.
Our results demonstrated the variations in the late restriction activities among closely related TRIM5α orthologues and a subset of human TRIM family proteins, providing further insights into the late restriction activities of TRIM proteins.
We also considered site variation in restrictions on the accrual and data collection process imposed by either the local research ethics board or the hospital.
Thus, these polymorphic SLAF markers were not suitable for genetic map construction.>> Exploiting the variation in restriction sites, enzyme digestion can produce fragments of different lengths in the two parents, and using SLAF-seq through gel extraction screening for fragments of a certain length, the same locus in the sequencing data will detect only one genotype, as shown in Fig. 2.
This restriction fragment length polymorphism (RFLP) results from variation in the size of a region of repetitive DNA 3' to the gene.
Variation in the frequencies of MHC alleles [ 12- 16], mitochondrial DNA (mtDNA) variation, restriction-site polymorphism (RFLP) haplotypes [ 17, 18] and sequence [ 19, 20], and Y-chromosome haplotypes [ 21] were also characterized.
