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Body shape diverged consistently in reservoir habitats in the replicated reservoir basins; however, there was substantial variation in the replicated stream populations in one reservoir basin (Thunderbird) where several stream populations were collected (Fig. 3).
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The standard deviation was calculated to show the variation in the replicates.
The SD was calculated to show the variation in the replicates.
Assay precision was inferred from the variation in the replicate Ct values for all genes used, and had no more than 5.3% coefficient of variation (CV).
The numbers of differentially up- and downregulated genes for every pairwise comparison are summarized in Additional file 2. In order to examine the degree of variation in the replicate expression profiles, principal component analyses were performed for the two time points separately.
Survival analysis was performed to determine whether variations in these replicated SNPs influenced the likelihood of death and hospitalisation due to cardiovascular illness.
The quantitative variation in the different replicates was calculated using the delta-delta threshold cycle relative quantification method.
Quantitative variation in the different replicates was calculated using the delta-delta threshold cycle relative quantification method.
The list of significantly (p < 0.05) changing proteins was triaged further by manual inspection of spectra and data, proteins showing consistent changes across patients and low variation in the technical replicates of the pooled standards were prioritised.
The actin gene was used as an internal control for data normalization, and quantitative variation in the different replicates was calculated using the delta-delta threshold cycle relative quantification method.
Figure 4D shows resultant images after 3 min of electrophoresis time, with dramatic band distortion and variation observed in the replicates with no glycerol present.
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