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Nevertheless, NIR-CI is able to show sinusoidal variation in intensities along the roller compacted ribbon among all settings studied.
The p53, p38 and p21 antibodies used in subsequent assays were tested by immunocytochemistry, but proved inadequate for quantification due both to a diffuse distribution throughout the cell body and cell-to-cell variation in intensities that appears to result from induction of stress pathways in some cells during transfection (data not shown).
The variation in intensities could be explained by batch, DNA hybridization, genetic group, and genotype (see also the paragraph on statistics in the Genomic DNA Analysis section).
However, achieving these goals is often quite difficult due to the large and complex nature of the datasets, and due to the biological and technical variation in intensities of spectral features.
Consequently when comparing crude extracts from identical cells cultivated in different conditions, we can assume that variation in intensities or intensity ratios may attest post-translational events or modification of the proteome being analysed.
High intra-probe set covariance between individual probe intensities indicate that 75% of probe sets were composed of functional probes whose variation in intensities robustly represented abundance of the intended target RNA species (Additional file 2).
Similar(54)
There is, however, a difference between variation in intensity and variation in volume of vocal tone.
Raman spectroscopy revealed variation in intensity of CNT's peak with variation in concentration of CNT.
The variation in intensity of specular beam during In deposition is reported in Fig. 2a.
Overall considerable variation in intensity of herbivore and pathogen damage was found throughout the range of groundsel bush.
That is to say, in image diffusion, the area with small variation in intensity has a large backward diffusion velocity.
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