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When we take a standard deviation of 0.15 as an approximate quantitative criterion (95% normal confidence interval, allowing a four-fold variation in expressions induced by amplification), for 39% of the genes the variation in the gene expression introduced by amplification was outside this confidence interval.
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They also used GWAS to identify loci associated with variation in expression of testis-expressed genes.
Levels of OsClpC1 transcript did not show much variation in expression; these transcripts were minimally expressed in endosperm.
Moreover, the expressed genes showed great variation in expression among different tissues and cultivars.
First, non-coding RNAs are often weakly expressed, which makes the detection of variation in expression harder.
Furthermore, in vivo analysis of Cherry-expressing mice shows up to 30-fold variation in expression between different tissue types.
Eight miRNAs were not differentially expressed and had a high degree of variation in expression levels (data not shown).
Interestingly, miRNAs expressed within a cluster also showed a large variation in expression.
Although this may explain the wide variation in expression of endometriosis, it cannot explain differences in our monozygotic twin.
There is inter-individual variation in expression however, miR-26b was significantly down-regulated in all but one primary lines.
For mRNA expression levels, predictive features are those mRNAs whose variation in expression levels is associated with survival outcome, and mechanistic features are those mRNAs whose variation in expression levels is associated with genomic variants.
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