Sentence examples for variation by sequencing from inspiring English sources

We investigated whether polymorphism of the coding regions of these genes could account for phenotypic variation, by sequencing the four genes of the two parental strains.

We screened for mitochondrial DNA variation by sequencing the cytochrome oxidase subunit I and II (COI-COII; 1368 bp) [GenBank: EU600788], the region of NADH dehydrogenase from subunit 4 to subunit 6 (ND4–ND6; 1497 bp with a gap of 100 bp) [GenBank: EU600793], and the region from ND6 to ND1, which includes the cytochrome b (2427 bp and 2430 bp) [GenBank: EU600789– EU600792].

To analyze these heterozygous sites, for each wAnaITG genome we attempted to phase the variation by sequencing a single F1 offspring of each wAnaITG containing strain (while absent of wAnaINF) of D. ananassae that had been crossed to a strain lacking both integrated and infectious forms of wAna (see Materials and Methods for details).

The common SNP/SNV (single nucleotide polymorphism/single nucleotide variation) calling algorithms generally use parameters such as read quality to evaluate and filter variations caused by sequencing errors.

Possible sequence variations were confirmed by sequencing the opposite strand.

Both SNP variation subclones were verified by sequencing.

Taken together, these findings demonstrate that analyses of genome variations by sequencing can shed important light on differences in responses of M. truncatula ecotypes to abiotic stress in general and mineral stress in particular.

Nucleotide variations were then determined by sequencing.

However, long reads are also a key in studying structural variations or investigating isoforms by sequencing full-length intact transcripts [ 8– 11].

Furthermore, plant species contain wide genetic variation that cannot be explored by sequencing only one or two plants from each species.

For all non-synonymous variations, the genotype was confirmed by sequencing an independent PCR product.