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In this region, we found a SNP variation at nucleotide position 18,317,741 (G to A) located on the second exon of LOC_Os10g34360.
To ascertain whether the variation at nucleotide level resulted in change in at amino acid level, the protein sequences of vp19 gene were also compared.
Evaluation of the administered vaccine by dual immunofluorescence staining showed ~2.5% variants with low or no RSV F expression while single nucleotide primer extension detected ~1% variation at nucleotide 2045 that resulted in a pre-mature translational termination at codon 85.
Alignment of sequence data from each group revealed the following results: there is a variation detected in MC4R-1 betheen the 2 groups at nucleotide 35 (T-G) (sense mutation) while we detected another variation at nucleotide 19 (T-C) in MC4R-2 gene (sense mutation) of low sexual desire does.
Three sheep (one Rambouillet, one Suffolk, one Katahdin) exhibited a missense C variation at nucleotide 377, H126P (haplotype m, Figure 2).
Our cDNA library contained ~2% clones coding for Q8UW25 isoform without any variation at nucleotide level.
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The variations at nucleotide and amino acid in the structural genes can result in difference in the protection offered by these proteins when used as vaccines.
The rationale behind this approach is the hypothesis that bacterial species are not a homogeneous group of organisms but contain significant individual variation at the nucleotide level (single nucleotide polymorphisms) and at the genome composition level (one or more genes, operons, and so on).
The level of genetic variation at the nucleotide level was estimated as nucleotide polymorphism (θ, [ 24]) and nucleotide diversity (π, [ 25]).
While identification of sequence variation identity at nucleotide resolution and position is valuable, determination of allelic imbalances (AI), defined as a shift from a 50 50 expressed ratio, require transcript sequencing of single samples.
Genetic variation at the nucleotide level was estimated from mean nucleotide diversity (πT = 0.0061) and from the number of segregating sites (θW = 0.0056).
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