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This method considers the interaction and interference between compliant parts due to part variation, assembly tooling variation, welding distortion, and spring back effects.
Discrepancies between the consensus sequences from these assemblies and the original consensus sequences were resolved as either intraspecific variation, assembly or alignment errors in the 40 bp read data set, or unresolved assembly errors.
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Despite this variation, during assembly a median value for each parameter is chosen and the assembler therefore is less optimal in those regions that differ from that median.
Dimensional variation in assembly processes is one of the most important issues that affect quality.
The variation in assembly results among isolates of the same species is depicted in Figure 6 A C.
The species and genera that were used to assess variation in assembly results among related genomes were selected based on the following criteria.
To provide an estimate of the reliability of this approach, the benchmark data were analysed to determine the variation in assembly results within recognised species and genera.
This information can be obtained by variation of assembly conditions such as concentration and solvents.
As neither sequence variation nor assembly inconsistency was found in the terminal contig, we concluded that the chromosome has inverted identical sequences at both extremities.
The variation in assembly quality between replicates was greater than between non-amplified and amplified libraries prepared by following either Phi or Tre protocols (Supplementary Table S2).
Trinity exhibited the most uniformity among assemblies compared to the variation among assemblies from Velvet/Oases and SOAPdenovo-Trans.
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