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To assess regional variation, analyses were performed by federal state.
Variation analyses were performed on natural logarithmic transformed data after exclusion of outliers using Cochran and Reed tests.
Differential co-expression and variation analyses were performed based on 30,035 and 12,364 probes from mouse ES cell and human iPS cell data, respectively.
The list of genes that distinguish infection from mock-infection defined by the SAM and variation analyses were further analyzed to identify biological themes associated with them.
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The trustworthiness of corresponding variation analyses is, however, not ensured by the availability of sophisticated methods and tools alone, but does evidently also depend on the accuracy of the input information used.
Co-variation analyses were performed using a recent implementation of the method of Carlson et al. [4].
To correct for allometric contribution towards shape variation, subsequent analyses were undertaken using the residual components as determined from the regression of shape on centroid size.
Recovery analysis was carried out on NDVI not corrected for rainfall variation, but these analyses were systematically implemented over time series of several years, in order to limit the influence of inter-annual seasonal variations.
To estimate the extent of genome size differences between strains, and the contribution of plasmids and the chromosome to this genome size variation, various PFGE analyses were performed.
For dates, we computed the coefficient of variation (C.V .. Analyses were performed using the SAS statistical software package (Release 9.1, SAS Institute Inc., Cary, North Carolina) and R [ 15].
As there was a significant difference in advertising pattern by seasonal variation, the following analyses were conducted separately for normal days and school holidays.
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