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The CIMRA assay alone correctly classified 65% of variants, with only 3% discordant classification.
Over the last few years, microarrays designed to cheaply screen human genomes for common genetic variations linked to common, complex diseases have mostly picked up variants with only a mild effect on disease risk.
To identify variants of this network that would accept and improve upon our best D469 mutants for activity towards PA, we created a library of random single, double and triple mutants across seven of the co-evolved residues, combining our D469 variants with only naturally occurring mutations at the remaining sites.
In this case, the setup is accomplished for a family of related product variants with only small or no setup requirements for a changeover to another variant in the same product family.
Analysis of the results showed that SOD activity increased (by 15%%) in variants with nanomolybdenum action and joint action of nanomolybdenum and microbial preparation, but in variants with only microbial preparation, it decreased by 20%% (Fig. 2).
These natural variants with only 7C or 6C in each domain form a new PI-II family within the PI-II superfamily.
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IRF-7C is an alternative splicing variant with only the DNA-binding domain of IRF-7.
Here, the resulting spectrum rather resembled that of the denatured Cys166Ala variant with only one covalent linkage.
By using a heterodimeric BMP2 variant with only one functional type I receptor epitope, we could confirm the presence and the number of these low affinity sites.
These receptor monomers bind wtBMP2, as well as the heteromeric wt/L51P variant with only one intact type I receptor site, with a lower binding affinity, resulting in a lower EC50 value.
The egc locus appears to be present in a variant or truncated form with only genes sem and seo being detectable.
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