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FDH variants with improved stability, expanded substrate scope, and altered regioselectivity have been developed.
Improvements in high-throughput screening methods have facilitated the rapid identification of variants with improved biosynthetic capabilities.
A hexokinase deletion strain was constructed to allow screening for variants with improved ability to transport xylose in the competitive presence of glucose.
We have now isolated Spy variants with improved ability to stabilize a poorly folded client protein (Im7 L53A in4A) in vivo.
These improved systems may allow the selection of Cry toxin variants with improved insecticidal activities that could counter insect resistances.
PON1 variants with improved γ-butyrolactonase activity.
PON1 variants with improved long-chain acyl esterase activity.
PON1 variants with improved acetoxy coumarin esterase activity.
Lin, J.Y., Lin, M.Z., Steinbach, P. & Tsien, R.Y. Characterization of engineered channelrhodopsin variants with improved properties and kinetics.
In addition, the substrate-surrounding residues were engineered to search for variants with improved enzymatic activity.
SDRO consisted of diversifying specificity-determining residues and selecting variants with improved affinity using phage display.
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