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Antibodies to NTS-DBL1α domains from other PfEMP1 variants, which do not recognize R29 live infected erythrocytes by IFA, also did not induce phagocytosis of R29 infected erythrocytes, even at high concentrations (Figure 9, control PAR+, TM180, TM284, HB3R+).
Those variants, which do not cause any amino acid change and already reported as polymorphisms in databases, were not considered to be significant and were excluded from further analysis (data not shown).
Other authors [ 29- 31] have described MUC1 RNA splice variants which do not express the protein core region.
While the functional effect of type 1 CFI genetic variants in the pathogenesis of AMD seems apparent, the effect of the rare genetic variants which do not result in a failure of secretion (type 2 variant) remains unclear.
In contrast, the 1 520 and Δ700 Cep126 variants, which do not contain the predicted centrosomal-localisation domain, did not localise to the centrosome and did not show detectable effects on MT organisation.
Additional problem variants which do not follow the exact templates presented here, such as the formulation in [ 12] for the RNA-RNA Interaction Partition Function problem, or the formulation in [ 13] for the RNA Sequence to Structured-Sequence Alignment problem, can be solved by introducing simple modifications to the algorithms we present.
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For completion, we also performed a further analysis of other possible heterozygous variants which did not reveal any mutation that could explain the biochemical and clinical features in this newborn.
The lower Moco content of the purified variants may be due to the modified purification protocol necessary for the purification of these mAOX1 variants, which did not bind to the benzamidine sepharose.
When we immunoprecipitated HA-tagged Nop14p with anti HA antibodies from the corresponding cellular extracts, corroborative results were obtained: HA tagged Nop14p could only coprecipitate Noc4p variants which did not lack its three C-terminal domains 6 to 8 (Fig. S1B).
3) The tested binding sites harbored nodal variants, which did not disrupt the prediction score of the TF-binding motif.
Other novel variants, which did not reach genome-wide significance, were identified for the different outcome measures, but need replication.
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