Sentence examples for variants were transformed from inspiring English sources
L1763 (strong [ PSI+][ pin-]), L2333 (sup35 mutant) or L2333 with [ PIN+] variants were transformed with plasmids (pNMPM-CSC, pNMCA-CSC, and control vector) and transformants were dissolved in water and spotted onto SD-Ura (to maintain the plasmid), low adenine (to monitor read through via color) or SD-Ura-Ade media (to monitor read through with growth on -Ade).
pMW119 and plasmids of acrB variants were transformed into electrocompetent JA300A.
DTA C186S (and its Lys-tagged variants) were transformed in Escherichia coli BL21 DE3) cells (Invitrogen).
For bacterial expression of proteins, pET23b vector containing cDNAs of either intact GFAP or its variants were transformed into the Escherichia coli BL21 DE3) pLysS strain (EMD Millipore).
Expression plasmids carrying the human CBS major allele, or variants, were transformed into this strain by selecting for uracil prototrophy (Ito et al. 1983).
To express Ms0025 and variants, BL21 DE3) star cells (Novagen) were transformed with the plasmid containing the gene.
For the production of BsDHFR and its variants, E. coli BL21 DE3) cells were transformed with the respective plasmid and grown to an OD600 of 0.6 in LB medium containing ampicillin (100 μg/mL).
Y. lipolytica ΔNUHM GB10 cells were transformed with pUB26 nuhm) variants as described previously [ 32] and selected on YPD [1% (w/v) yeast extract/2% (w/v) peptone/2% (w/v) glucose] agar plates with 100 μg·ml−1 hygromycin B at 30°C.
For the TDP-43 strains, yeast were transformed with linearized pAG303GAL-TDP-43WT or its variants.
BL21 cells were transformed with pDIMC8 plasmids containing genes that encode the protein variants.
