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As a control, a fraction of variants were sequenced using the ABI Prism BigDye Terminators and the ABI Prism 3100 sequencer (Applied Biosystems).
Virus variants were sequenced and plasmid-based reverse genetics was used to examine the effect of each mutation.
Over 600 variants were sequenced to compile a comprehensive database of P8 sequence diversity compatible with assembly into the wild-type phage coat.
Noteworthy, the alignment-based approach that was used for the calculation of the total mean sequencing error rate revealed that several MinION reads were composed of non-contiguous regions of the YCNV genome, suggesting that either defective viral variants were sequenced or that the MinION generated chimeric sequences.
The peptides from the H2A variants were sequenced with different PTMs (Table 3, Figure S2A).
The scFv gene fragments of the improved variants were sequenced (figure 1).
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Viral DNA encoding nerve agent model compound-resistant hBChE variants was sequenced to identify sites where mutagenesis was present.
While the sequencing coverage is high enough that even rare viral variants are sequenced, the presence of sequencing errors makes it difficult to distinguish between rare variants and sequencing errors.
Sixteen individuals who were found to be enzymatically deficient but were genotyped as wild-type for the 202 variant were sequenced to assess other alleles that define the G6PD A- variant (G680T and T968C).
Additionally, 72 randomly selected samples negative with RFLP-PCR for the C5972T variant were sequenced.
Having become the largest sequencing center in the world over the past year, the BGI has sequenced 540 plant and animal reference genomes and 25,239 variant genomes, including 38,123 human samples (multiple samples of one variant were sequenced).
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