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These variants were prepared by using another forward primer with an additional ATG start codon, processed and tested in parallel with the other thiolases.
A series of minireplicon variants were prepared with copy number ranging from low (1 2 copies per chromosome), medium (10 15 copies per chromosome) to high copy number (80 90 copies per chromosome).
For that reason, several intermediate variants were prepared and tested for antigen-binding activity.
The library variants were prepared with a His6 tag for ease of purification.
The GST-fusion recombinant proteins of survivin variants were prepared as previously reported [ 15].
A number of Mja KlbA site-directed variants were prepared to determine the best site for peptide ligation (Supporting Information).
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Phage display is a process in which large libraries of protein or peptide variants are prepared as fusion proteins with one of several coat proteins on the surface of bacteriophage.
Co III) complexes of DPP (DPP = (Z -2- 2- 5- 2-hydroxyphenyl -1H-pyrrol-2-yl)(phenyl)methylene)-2H-pyrrol-5-yl)phenol) and two fluorinated DPP variants are prepared, structurally characteriZ -2- 2- 5- 2-hydroxyphenyl -1H-pyrrol-2-yltalytic ring-opening of 1,2-epoxyhexane with alcohols.
A library of DERA variants was prepared using error-prone PCR.
The newly characterized enzymes AdhZ2 and AdhZ3 should be considered, when knockout variants are prepared that are not supposed to reduce aldehydes, as for example shown in the production of isobutyric acid (Zhang et al. 2011).
The protein structural model for the localization of the TH variants was prepared based on the human TH X-ray diffraction structure (PDB 2XSN, a truncated form of the enzyme lacking the N-terminal first 192 residues and which was crystalized with a Zn2+ replacing the Fe2+ in the catalytic site).
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