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Until recently, domestication of clonally propagated crops has been considered a simple process dubbed 'single-step domestication' in which spontaneous variants were picked up by early farmers and since then propagated vegetatively (Zohary, 2004).
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Single colonies of Escherichia coli BL21-containing plasmids encoding either wild-type (control) or variant arginase enzymes were picked into 96-well culture plates containing 75 μL of TB medium/well and 50 μg/mL kanamycin.
From the four samples, 240 SNPs and 240 small InDels were picked for variant validation by Sanger sequencing.
Colonies were picked and stocked.
Selected spots were picked with a 2 mm picking head.
were picked for maturity analysis.
Clones were picked based on morphology.
F1 cross progeny were picked and cloned.
Positive clones were picked and grown.
Single colonies were picked and expanded.
G418-resistant clones were picked and established.
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