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Several variants were obtained with higher purification yields than even sBBI.
Molecular details about the function and structure of some variants were obtained by enzyme kinetics and circular dichroism.
For most of the inhibitors studied, progressive loop replacement at the trypsin surface resulted in inhibitory profiles akin to factor Xa. Crystals of the variants were obtained in the presence of benzamidine (3), and could be soaked with the highly specific factor Xa inhibitor (1).
Following intra-nasal delivery, low cerebral hemisphere levels of variants were obtained at 20 min, with a trend towards faster clearance of the high FcRn binder (N434A); however, the relatively higher serum levels confounded analysis of brain FcRn contribution to efflux.
These variants were obtained using either the Taq or Herculase DNA polymerases, after one or two rounds of PCR (Table 1; Figure S1).
Using the two state-model eqn 1 [16] (experimental) the transition state parameters of the three GST-C variants were obtained (Table 1).
Similar(17)
After three rounds of mutagenesis and screening, a diverse set of tighter binding variants was obtained.
The highest number of improved BSLA variants was obtained for DMSO (126) followed by DOx (62) and TFE (62) (Fig. 3a c).
Required software diversification among variants is obtained using off-the-shelf cross-compilation suites, whereas hardware diversification relies on processor emulation.
Then, in the framework of irreversible thermodynamics, a kinetic relationship, a martensitic nucleation criterion and the reorientation criterion of martensitic variants are obtained.
By comparing 5 different donors a similar pattern for the relative ratios of FOXP3 splice variants was obtained (Fig. 2B).
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