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The high temperature phase of Ta2O5 and its variants were maintained in a pure Ta2O5 specimen using the conventional solid-state reaction method and the advanced laser irradiation technique.
Stock Opa variants expressed LOS species with the same banding pattern on silver stained tricine gels (data not shown) and stocks composed of either mostly piliated or nonpiliated variants were maintained.
The resulting variants were maintained in culture under adhered conditions.
SV40-immoratalized MEF WT and its variants were maintained in DMEM containing 10% FBS and antibiotics.
BT-20 cells, MCF-7 cells and their pulse-selected variants were maintained in MEM supplemented with 10% fetal calf serum (FCS), 1 m M sodium pyruvate and 1 m M non-essential amino acids.
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After transmission of a CTL escape variant to a new host who does not carry the restrictive HLA allele, some variants are maintained while others revert, suggesting that those which revert are associated with a decrease in viral fitness [21], [34], [35].
The findings show that in WFDC6 all NSyn variants are maintained a very low frequencies and that the fixed difference K79E, predicted to alter protein function in EPPIN, is also present in bonobos (>1 Ma).
To assay MUSK-induced motor endplate maturation, fetal myocytes from a fetus homozygous for the c.1724T>C; p. (Ile575Thr) variant were maintained in DMEM supplemented with 20% fetal bovine serum, glutaMAX and penicillin/streptomycin at 37 °C in 5% CO2.
Upon treatment of MCF-7 cells with cisplatin and the estrogen receptor antagonists 4-hydroxy tamoxifen and ICI 182780, endogenous protein cyclin D1a expression is strongly reduced, whereas oncogenic cyclin D1b splice variant is maintained and confers chemoresistance [ 103].
The multiplicity of infection (MOI) of the drug-resistant 2C-N179G variant was maintained at 10 plaque-forming units (PFU /cell while that of the drug-susceptible virus ranged from 0too 100 PFU/cell.
The genetic isolation of the FuI population and the predominance of early non-lethal mutant phenotypes indicated that the genetic variants responsible for the phenotypic variation are maintained due to population isolation.
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