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By a novel genetic selection/screening process, two BstYI variants were isolated with a preference for AGATCT cleavage.
Importantly, non-reducing SDS-PAGE gels showed that the cysteine variants were isolated predominantly as monomers.
Heads of flies with pan-neural expression of different TDP-43 variants were isolated using Trizol (Invitrogen).
Five of the Manitoba NA S286G variants were isolated during outbreak weeks 08, 09, and 11 and were associated with severe disease.
From all recipients additional clonal HIV-1 variants were isolated between 9 and 22 months from a time point 54 112 months after transmission.
Resistant variants were isolated by culture in presence of increasing chloramphenicol concentrations, as previously used for selection of antibiotic-resistant E. aerogenes strains [31].
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Unlike amantadine, it takes several passages in culture before resistant variants are isolated.
However, many variants are isolated only rarely or from a single country and are not transmitted further because of bottlenecks associated with geographic spread and competition (19, 22 ).
Human His BCNT and two bovine His-variants were isolated from E. coli and acetylated using recombinant CREB-binding protein (CBP, 2 μg) and C-radioactive acetyl-CoA (0.3 μCi, specific activity 3.7-4.44 GBq/μmol, ARC0554) as described previously [ 15].
When the heterokaryotic parent was paired with the homokaryon A, a variant was isolated from the tuft.
A new circadian variant was isolated by screening the intercross offspring of wild-caught mice (Mus musculus castaneus).
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