Sentence examples for variants were inserted from inspiring English sources

All of these 7ZFPs and their variants were inserted into T-cloning vector and confirmed by sequencing.

Both the wild-type spo0A and the synonymous spo0A variants were inserted with a downstream selection marker.

For recombinant protein expression in bacteria, inserts for the Rab26 variants were inserted into pGEX-KG using EcoRI and BamHI while the insert encoding alpha-GDI was sub-cloned into pET-28a (Novagen, Madison, WI).

All CipA variants were inserted into the C. acetobutylicum genome by Allele-Coupled Exchange (ACE) technology as described by Heap et al. [ 21], using the pMTL-JH16 integration plasmid.

The polymerase chain reaction products were then inserted into the pMD-18T simple vector to generate pMD-18T-IL3 vandanthe aNotIhe NotI– NotI fragments from pMD-18T-IL3 variants were inserted into the pET-28a-sCARL plasmid to generate plasmid pET-28a-sCAR-IL3.

Each of the five MjTyrRS variants was inserted into the pMV361 vector and co-transformed with pSMT-MjtRNA-GFP151TAG into M. smegmatis.

The histone variant H2AZ and likely other histone variants are inserted into assembled nucleosomes by histone variant exchange complexes (HVE) such as SWR1.

Epitope prediction was performed to determine whether or not the genetic variants are inserted in B-cell and T-cell epitopes.

PCR fragments of the CaV2.2 long splice (cdB1) variant was inserted into the TA cloning vector pCR2.1 (Invitrogen) and cut out at EcoRI and XhoI sites, then subcloned into pGEX-KG (GE healthcare), pPr-IBA (IBA; OneStrep tag), or pGEX-KG with a sub-cloned FLAG tag (to generate GST-FLAG tagged constructs) expression vectors.

FP reporters ECFP, mCherry, and the YFP variant, Topaz were inserted into Trap, Dmp-1 and Ibsp, respectively.

In the cases of variants 1 6, no linkers were inserted between the C-terminal sequence of mcpGFP and the second part of AMT1 3.