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The Su(H) variants were injected at the 1 2 blastomere stage and harvested at the 3-somite stage for in situ hybridization with a her8a riboprobe (Fig. 3F H).
Affinity maturation variants were injected onto the IL-6R surfaces at concentrations up to 200 nM.
Recombinant hIL-6R was captured at 100 nM, and the different Nanobody variants were injected onto the B-N12/IL-6R surfates at concentrations between 6 and 200 nM.
Expression constructs for lin-11p variants were injected at 30 ng/μL along with unc-122p :: mCherry (10 ng/μL) as a transformation marker and pPD49.26 (60 ng/μL) as carrier DNA except that lin-11pAΔ:: venus was injected at 30 ng/μL along with tdc-1p :: mRFP (30 ng/μL) for marking RIC neurons and pRF4[ rol-6 (d)] (40 ng/μL) for inducing roller phenotype.
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A 100 μl aliquot of rhTRAILWT and variants was injected at concentrations ranging from 1 to 250 nM at 50 μl/min and at 37°C using HBS-N supplemented with 0.005% surfactant P20 as running and sample buffer.
Human wild-type and variant GREM2 mRNAs were injected at various concentrations ranging from 2 to 5 pg/nl.
Two LBV isolates from South Africa (LBVSA2004) (9) and the LBV mongoose isolate described in this report (Mongoose2004), as well as a North American bat RABV (Myotis spp. variant, isolated in Washington, USA, 2004), were injected into 4-week-old inbred ICR mice (5 mice/group) by different routes.
BubR1 (wild type, variants and the phosphopeptide) at a concentration of 0.7 mmol/L were injected in 1.5 µL quantities every 120 s for a total of 20 injections into B56γ1 samples (wild type and variants).
In control experiments for possible mass transfer limitations, the IgGs were injected over the receptors and the EndoS variants over the IgG sub-classes at different flow rates.
Embryos were injected with a helper plasmid encoding the most active variant, Int*-KEE.
One-cell fertilized zebrafish embryos were injected with varying amounts of DD/RR or EL/KK FokI variant mRNAs.
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