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Furthermore, we review how these variants were initially designed and subsequently optimized by spontaneous viral evolution.
Seven variants were initially designed to change the preference from xylose to glucose at substrate binding subsites −2 and −1.
These variants were initially identified in a subset of DBA patients and later in healthy individuals but at a low frequency [14], [15].
Serovars and variants were initially identified by their RFLP patterns after polyacrylamide gel electrophoresis.
For diploid F. iinumae, variants were initially discovered by specifically mining the F. iinumae F1D VCF file (Additional file 9).
These persisting first-wave variants were initially detected on February 13 , March 23 and August 24 , 2012 and they were still being detected in December 2012 (during the second infection wave), the last month of the surveillance reported here.
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Rescued variants are initially classified as nonsense or missense, but switch their class to same sense or missense after the RMA correction.
This becomes especially important in next generation sequencing projects, where typically thousands of genomic variants are initially identified.
Prn2 and Prn3 emerged in the 1980s, 20 to 30 years after the introduction of vaccination and although the frequency of the two variants was initially similar, Prn2 is now by far the most predominant type.
Variants are initially subjected to Affymetrix quality control as described by Affymetrix [ 26].
As with the symptomatology analysis, association with STS variants was initially tested in males only (Table 3).
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