Eight SCF variants were expressed and purified.
The genes, Aoxyn11A and its variants, were expressed in P. pastoris GS115.
The chitinase variants were expressed in E. coli using a prokaryotic signal peptide, PelB, for efficient secretion.
The sIGPS protein and its variants were expressed in BL21/DE3 cells and purified as described previously [17].
Different truncated fly-rhodopsin Rh1 variants were expressed in the eyes of Drosophila and their localization was analyzed in vivo or by immunofluorescence.
Antibody wild type and variants were expressed at 10 100 mg levels by transient transfection of Freestyle HEK 293 cells (Invitrogen) with polyethyleneimine (Polysciences) as the transfection reagent.
Each one was mutated into alanine and the resulting single amino acid variants were expressed, purified and tested for activity against chimeric substrates (Fig. 3B).
Sorting the cells for low GFP levels yielded populations where the p130Cas variants were expressed to levels similar to endogenous p130Cas in Cas +/+ MEFs (Fig. 1B).
Mutants YFP-SERT variants were expressed in CHO cells and their distribution was compared with that of Texas Red conjugated wheat germ agglutinin, a lectin marker for the plasma membrane.
