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Using a commercial software, Kd constants measured for all three PAB variants were estimated to be 40 ± 6 nmol/L and 72 ± 25 for PAB036, 49 ± 10 nmol/L for PAB046, and 10 ± 3, 12 ± 3 and 21 ± 8 nmol/L (an average value 14 nmol/L) for PAB050.
The correlations between the genetic variants were estimated from the EPIC-InterAct data.
The relative copy number of the 10 sires for KIAA1683, and its V1 and V2 variants were estimated using qPCR.
Antigenic shifts and reappearances of past variants were estimated to have pandemic potential every 8 10 years (31, 32 ).
Finally, the ages of these variants were estimated using a coalescent-based method implemented in the GENETREE package.
Protein stability changes due to variants were estimated by MUpro [ 31] which estimates both energy changes and the reliability of underlying prediction.
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As a master regulator of innate and adaptive immunity, NF-κB plays a critical role in autoimmunity, including in MS, where 18% of all allelic MS risk variants are estimated to affect or intersect with the NF-κB signaling pathway4.
The CSPG binding capacity of the two DBL5ε variants was estimated by ELISA.
The time to the most recent common ancestor (tMRCA) for these HBV/E variants was estimated to be year 1952 (95% highest posterior density (95% HPD): 1927 1970).
The divergence time of the Japanese black bear DQB variants was estimated using synonymous substitutions alone.
The population frequency of all novel variants was estimated using PCR RFLP or HRM tests.
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