Sentence examples for variants were done from inspiring English sources

Identification, verification and annotation of sequence variants were done using GeneScreen software (http://dna.leeds.ac.uk/genescreen) [ 36].

Identification of different γ variants was done using an original approach based on automatic analysis of Kikuchi patterns obtained by TEM in convergent beam mode.

Structural superposition of the Erg8, Erg9 and HFA1 protein product variants was done using the SuperPose.

Also, since two non-synonymous variants (rs3743930, rs224222) as well as three synonymous variants (rs224225, rs224224, rs224223) were observed in MEFV exon 2 during the sequencing screening phase, genotyping of these variants was done using a sequencing approach.

Validation of variants was done with Sanger sequencing.

Expression of WT proteins and variants was done as described for p66CH2CB [ 30].

Subsequent analysis of variants was done within R using a custom-made R library flowgram.

Thorough bio-informatic evaluation of novel variants was done using Alamut software (v.1.5).

Annotation of variants was done with in-house developed software which uses Ensembl API and Alamut Batch, while filtering of relevant variants was done using in silico tools as specified below.

Prediction and understanding of the downstream effects of the nonsynonymous and noncoding variants was done using computational methods.

Computational analysis to detect novel single-nucleotide variants was done as previously described (Andrews et al., 2012).