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Gβ3 allelic variants were determined in each subject using a PCR-based technique.
TPH allelic variants were determined in each subject using a PCR-based technique.
SERPR allelic variants were determined in each subject using a PCR-based technique.
Stability changes for the toxin variants were determined using circular dichroism and fluorescence measurements, and scanning calorimetry.
The kinetic rate constants and equilibrium dissociation constants for monomeric and dimeric ecotin variants were determined with both trypsin and chymotrypsin.
The K d values of substrates to the CYP102A1 variants were determined (at 23°C) by titrating 2.0 μM enzyme with the ligand, in a total volume of 1.0 ml of 100 mM potassium phosphate buffer (pH 7.4).
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The relative abundance of the various VEGF splice variants was determined by RT-PCR using cDNA available in our laboratory.
The size and morphology of a critical nucleus, comprising of one or more variants, are determined by the competition among bulk thermodynamic driving force, elastic strain energy, and interfacial energy.
Our key insight is the firm's optimal number of product variants is determined by a parameter which is a function of market size and operations-related costs such as setup and inventory holding costs.
Native molecular mass of MtbMfd and its variants was determined by gel filtration chromatography.
Using subcellular fractionation, the cellular localization of wild-type and PepD variants was determined.
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