S. Typhimurium and its variants were detected rapidly and accurately.
For three loci14,17,24 only two allelic variants were detected.
Copy-number variants were detected with three steps, by comparison with actual coverage of the resequenced A. thaliana reference strain Col-0.
Twelve variants were detected only in the FFPE data; all were SNVs and indels and 7 of 12 had an AF lower than 0.13.
In contrast, 78% of the pathogenic variants were detected by combined analysis of WES and RNA-Seq, which represents the current gold standard for precision oncology.
Both variants were detected within each breed with the exception of Hereford, and the heterozygosity varied between 0.000 and 0.524.
Finally, a total of 1,223 genes with average 2.8 splicing variants were detected among 16,540 unique genes.
In five infants, the viral population arose from a single variant, while two variants were detected in the remaining infant.
RESULTS: Twenty-two coding variants were detected across the COL8A2, SLC4A11, and ZEB1 genes; six were nonsynonymous variants.
However, several size variants were detected of NecC produced by T. discophora under native conditions.
No escape variants were detected after therapy.
