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Single chain TetR variants were constructed that contain DNA reading heads with two different operator binding specificities.
Libraries of variants were constructed and screened on sucrose, allowing the identification of a mutant that showed a 6-fold enhanced activity toward sucrose compared to the wild-type enzyme.
Using the parental plasmid pET11a-ssh10b, thexpressionon plaimid of the Mth10b variants were constructed.
NAS and NBS cDNA variants were constructed using inverse PCR-based mutagenesis.
Rationally designed, site-specific PEGylation variants were constructed by orthogonal maleimide-thiol coupling chemistry.
In order to determine the ideal Gcg-XTEN for overnight administration, four length variants were constructed.
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A set of nine catalytically diverse P450 BM3 variants was constructed and tested toward the native substrate-inspired fluorogenic substrate 12- 4-trifluoromethylcoumarin-7-yloxy dodecanoic 12- 4-trifluoromethylcoumarin-7-yloxy dodecanoic 12- 4-trifluoromethylcoumarin-7-yloxy dodecanoic 12- 4-trifluoromethylcoumarin-7-yloxy dodecanoic 12- 4-trifluoromethylcoumarin-7-yloxy dodecanoic
A library of enzyme variants was constructed by staggered extension process (StEp) using the genes that code for the l-ASNases from Erwinia chrysanthemy (ErL-ASNase) and Erwinia carotovora (Ecal-ASNase) and screened using activity assays.
Subsequently, an alternative splicing graph of potential splice variants was constructed.
A network of MHC variants was constructed with the Neighbor-Net method [ 75] by using Splits-tree4 ver. 4.11.3 [ 76].
A library of polymerase variants was constructed in which amino acid residues 746, 747, and 748 were completely randomized.
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