Sentence examples for variants were confirmed from inspiring English sources

These variants were confirmed by Sanger sequencing.

All putative candidate variants were confirmed with targeted amplicon sequencing, a method with a sensitivity of 0.5% (ref. 14).

The bioinformatics pipeline was also run using CD8+ cells as germline control, but no somatic variants were confirmed in subsequent sequencing with the deep Amplicon method in CD4+ cells.

We suspected that the potential causal or susceptibility variants might be located within this range.11 As rs2254298 shows the strongest signal and is the most well- replicated SNP in this region, we first focused on novel findings near this SNP and four novel variants were confirmed by Sanger sequencing.

Candidate deep intronic cryptic splice variants were confirmed by in vitro analysis with expression minigenes containing relevant introns.

The MME variants were confirmed by Sanger sequencing of the entire MME coding region in all CH patients; no additional rare variant was detected except for a synonymous SNP (rs200455903).

Somatic variants were confirmed using Sanger sequencing and recurrently mutated genes were assessed in 13 additional PCs as well as 40 parathyroid adenomas (PA).PC had an average of 51 somatic variants/tumor (range 3-176) with approximately 58% of variants occurring as nonsynonymous single nucleotide variants.

Putative variants were confirmed by traditional Sanger sequencing of fresh LR-PCR amplicons.

All sequence variants were confirmed by sequencing the products of independent PCR reactions in both directions.

Coding variants were confirmed on repeat PCR reactions to exclude PCR artifacts.

Point variants were confirmed by DNA sequencing.