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After an overnight growth at 37 °C, colonies harbouring the epCelStrep encoding gene variants were collected by adding liquid LB medium containing ampicillin (100 μg/mL) to the plate surface and by suspending them with a scraper.
Non-redundant exons of all transcript variants were collected.
Variants were collected from both our internal data and public data.
The Mendelian disease variants were collected from OMIM and ClinVar, and the promoter elements from the FANTOM5 project.
Interestingly, previous research noted that different colour forms occurred in the Torres Strait, where dark and light colour variants were collected from Saibai (Allsopp 1991).
Predictions of gene structure variants were collected and organized from the ECgene web-site [ 43] and database [ 14, 15] and from the NCBI AceView database [ 41], based on EST and SAGE expression data.
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The 4 samples positive for the novel HEV variant were collected during 2008 2011 from herds across Jutland, Denmark, with a minimum distance of 80 km between the herds.
The flow-through (containing pure hArc protein, either WT or V231G hArc variant) was collected.
When available, CPT1A genotype data for the P479L variant was collected for unexpected infant death cases (SIDS/SUDI and death due to infection) where sub-optimal CPT1A function might influence outcome.
The sample size is lower for those variants that were collected with the fingerprint chip only (all VNTRs, rs1800955, and rs1611115) compared to the remaining variants that were derived from the fingerprint chip and complemented with imputed data, or derived from the imputed data only.
Gene transcript and variant data were collected and mined for candidate STEPs in motif regions.
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