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Four road alignment variants were analysed using the AHP, SAW and TOPSIS methods, with different sets of criteria weights and various preference scenarios.
All new and retested variants were analysed using the TA luciferase reporter assay as previously described.13 Briefly, BRCA1 constructs were co-transfected in HEK293FT cells with the pG5Luc plasmid, encoding a Luciferase reporter gene driven by GAL4 binding sites, and the phGR-TK plasmid, which constitutively expresses the internal control Renilla luciferase.
All ∼200 000 iCOGS variants were analysed with TL, and those displaying associations in healthy controls (n = 15 065) were further tested in breast cancer cases (n = 11 024).
Furthermore, all variants were analysed using the same genetic model as in the primary study, but none of the variants gave stronger association with the quantitative traits.
Intracellular localization, trafficking and protein association of GFP-tagged HFE protein variants were analysed in transiently transfected HepG2 cells by immunoprecipitation and immunofluorescence assays.
However, putatively truncating mutations (e.g. new stop codons or modifications within essential splice sites, and indel variants) were analysed for the remaining individual of this family (see Table S1).
Similar(32)
Each of the nine variants was analysed in a case-control study of type 2 diabetes including 3,612 Danish cases and 4,973 control subjects (Table 1).
mRNA folding of all the KLK15 variants was analysed by in silico modeling using mfold version 2.3.
The in vitro expressed S-labeled actin variants are analysed in various ways: on denaturing or native gels, in a band shift assay or in a copolymerization test.
The frequency of the intron 4 variants was analysed in 357 individuals divided in two control and two cancer patient groups.
To evaluate whether variants in ABCA4 jointly (common and rare; protective and adverse) are associated with toxic maculopathy, the genotype matrix containing the identified ABCA4 variants was analysed with a SNP-set (Sequencing) Kernel Association Test SKATT) in R [ 19] using the functions SKAT_Null_Model and SKAT_CommonRare with standard settings from the package "SKAT" [ 20].
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