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In addition, the same variants were active against both Heliothis zea and S. exigua [ 20].
All of the labeled variants were active, inhibiting bovine trypsin with an SI of 1.1 similar to that of the wild-type.
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Transcription of c-KIT and both SCF splice variants was active in normoxic controls and cells subjected to hypoxia followed by a 24 hour recovery while maintained in medium with or without recombinant rat (rr) SCF (100 ng/ml) (figure 6C).
Here, we demonstrate that the potentiated Hsp104 variants are active against a broad range of disease-linked TDP-43, FUS and α-syn mutants.
Taken together with the localization analyses these results suggest that the truncated Ilv2 versions as well as the Ilv5ΔN48 and the Ilv3ΔN19 variants are active enzymes even outside the mitochondrial matrix.
However, data for the LB03 H229andnd LB03 E73A mutants suggest that these enzyme variants are active and aqueous-soluble in the periplasm, albeit at slightly less abundance than the enzyme from their parent strain LB03.
Under these less stringent conditions we found three additional variants that were active (1 2, 1 6 and 1 12).
Surprisingly, some small deletions and point mutations resulted in altered regulation as these variants were moderately active when glucose was depleted, without requiring the inductor methanol [ 41].
In fact, in the Discussion you state you're surprised that all Spy variants were more active in aggregate prevention of Aldolase and α-Lactalbumin, but why is this a surprise since they only selected for improved chaperone activity.
For some of the substrates (most notably 12-pNCA and 2PE), many of the P450 variants were sufficiently active to either saturate the substrate or exceed the linear range of absorbance readings.
As both Cys316Ser and Cys316Ala variants were less active for glycine adenylation than wild-type AlmE, it appears that substituting smaller side chains removes optimal packing interactions maintained between native AlmE and the Cα of the glycyl group.
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